TERT Antilichaam

Telomerase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. The enzyme consists of a protein component with reverse transcriptase activity, encoded by this gene, and an RNA component which serves as a template for the telomere repeat. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks.

Western blot: 1:1000-1:2000, Immunohistochemistry (FFPE) : 1:25-1:50, Flow cytometry: 1:25 (1x10e6 cells), Immunofluorescence: 1:25

O14746

Human

A portion of amino acids 1104-1132 from the human protein were used as the immunogen for the TERT antibody.

WB, IHC-P, FACS, IF

Antigen affinity purified

Purified

This TERT antibody is available for research use only.

Aliquot the TERT antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

In 1X PBS, pH 7.4, with 0.09% sodium azide

The stated application concentrations are suggested starting points. Titration of the TERT antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Cytoplasmic

Western blot testing of human 1) Raji and 2) HL60 cell lysate with TERT antibody. Predicted molecular weight: 120-126 kDa (two isoforms may be visualized) .