Tetrahydrobiopterine ELISA Kit

THB, Tetrahydrobiopterin, THB, BH4

All Species

Tetrahydrobiopterin (BH4, THB, trade name Kuvan) or sapropterin (INN) is a naturally occurring essential cofactor of the three aromatic amino acid hydroxylase enzymes, used in the degradation of amino acid phenylalanine and in the biosynthesis of the neurotransmitters serotonin (5-hydroxytryptamine, 5-HT), melatonin, dopamine, norepinephrine (noradrenaline), epinephrine (adrenaline), and is a cofactor for the production of nitric oxide (NO) by the nitric oxide synthases.

ELISA Kit

Enzyme-linked Immunosorbent assay-Competitive

Aviva Systems Biology Tetrahydrobiopterin ELISA Kit (OKEH02612) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Tetrahydrobiopterin antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Tetrahydrobiopterin and incubated. The Tetrahydrobiopterin found in the sample or standards competes with the biotinylated Tetrahydrobiopterin for limited binding sites on the immobilized anti-Tetrahydrobiopterin antibody. Excess unbound biotinylated Tetrahydrobiopterin and sample or standard Tetrahydrobiopterin is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Tetrahydrobiopterin captured in the well and inversely proportional to the amount of Tetrahydrobiopterin which was contained in the sample or standard.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids

0.156-10ng/ml

Mean recovery when spiking into Serum and Plasma = 100%

0.067 ng/mL

Store as indicated in product manual.

Natural and recombinant General Tetrahydrobiopterin

Tetrahydrobiopterin