TF-1 Lysaat

TF-1 cells have been derived from a patient with erythroleukaemia. TF-1 cells are completely dependent on GM-CSF or IL-3 for long term growth. TF-1 cells respond to a variety of other lymphokines and cytokines such as IL-1, IL-4, IL-6, IL-9, IL-11, IL-13, SCF, LIF and NGF. TF-1 cells do not express glycophorin A or carbonyl anhydrase I.

Human

WB

2 mg/mL (determined by BCA assay)

TF1, ATGL0006-10 µg, ATGL0006-20 µg, ATGL0006-50 µg, ATGL0006-100 µg, ATGL0006-250 µg, ATGL0006-500 µg, ATGL0006-1 mg, ATGL0006-10, ATGL0006-20, ATGL0006-50, ATGL0006-100, ATGL0006-250, ATGL0006-500, ATGL0006-1

Aliquot the lysate into single tube and then storing them -80C or below. Avoid repeated freezing and thawing cycles.

Liquid in. SDS-PAGE Sample Buffer (0.12M Tris-HCl buffer pH6.8, 0.4% SDS, 5% glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)

Lysates

Human

All lanes : TF-1 Whole Cell Lysate (40ug) ; ; ; ; Lane 1. : Anti-GAPDH antibody (ATGA0181) ; ; Lane 2. : Anti-Beta tubulin antibody (ATGA0196)

Heat lysate to 95°C for 5 minutes and rapidly cooling before use.

Western Blot analyzed by both GAPDH and Beta tubulin antibody