TF-eiwit (inheems, menselijk)

TF

HEL-S-71p, PRO1400, PRO1557, PRO2086, TFQTL1

Native

< 1.0 EU per μg protein as determined by the LAL method.

≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

1.Immobilized Recombinant Human Transferrin Protein (Cat: 11019-HNAH2) at 2 μg/mL (100 μL/well) can bind Recombinant Human TFR1/CD71 Protein (ECD, AVI & His Tag), Biotinylated (Cat: 11020-H44H1-B), the EC50 is 30-90 ng/mL (QC tested) . 2.Recombinant Human Transferrin Receptor / TFRC / CD71 Protein (ECD, His Tag) (Cat: 11020-H07H) captured on CM5 chip via Anti-His antibody, can bind Recombinant Human Transferrin Protein (Cat: 11019-HNAH2) with an affinity constant of 63.500 nM as determined in an SPR assay (Biacore T200) (Routinely tested) . 3.Recombinant Human TFR1/CD71 Protein (ECD, AVI & His Tag), Biotinylated (Cat: 11020-H44H1-B) captured on CM5 chip via Anti-His antibody, can bind Recombinant Human Transferrin Protein (Cat: 11019-HNAH2) with an affinity constant of 75.510 nM as determined in an SPR assay (Biacore T200) (Routinely tested) . 4.Immobilized Recombinant Human Transferrin protein (Cat: 11019-HNAH2) at 2 μg/mL (100 μL/well) can bind Recombinant Human Transferrin Receptor/TFRC Protein (His Tag), Biotinylated (Cat: 11020-H07H-B), the EC50 is 646.8 ng/mL (Routinely tested) .

Val20-Pro698

The recombinant Human TF consists of 679 amino acids and predicts a molecular mass of 75.19 kDa. It migrates as an approximately 79.4 kDa band in SDS-PAGE under reducing conditions.

Human