TFPI-eiwit (C-His, mens)
Kwaliteit
ISO Gecertificeerd
Levering
24-48 uur
Technische Specificaties
TFPI
EPI, LACI, TFI, TFPI1
• HEK 293
C-His
< 1.0 EU per μg of the protein as determined by the LAL method
> 85 % as determined by SDS-PAGE
Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK (Dnp) -NH2 (Anaspec, Catalog#27114) . The IC50 value is < 0.4 nM, as measured in 100μL reaction mixture containing 1.25 ng trypsin (Sigma, Catalog#T4799), 10 μM substrate, 50 mM Tris, 10 mM CaCl2, 0.15 M NaCl, 0.05% Brij-35, pH 7.5 .
Met1-Lys282
The secreted recombinant human TFPI comprises 265 amino acids with a predicted molecular mass of 30.6 kDa. As a result of glycosylation, the apparent molecular mass of rhTFPI is approximately 42-45 kDa in SDS-PAGE under reducing conditions.
Human
Beschrijving
TFPI-eiwit (C-His, mens) Beschikbaar in 100 µg. Bestel eenvoudig online met snelle levering.
Specificaties
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