TFPI-eiwit (C-His, Mouse)

TFPI

A630013F22Rik, EPI, LACI, Tfpi

C-His

< 1.0 EU per μg of the protein as determined by the LAL method

> 97 % as determined by SDS-PAGE

Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK (Dnp) -NH2 (Anaspec, Catalog # 27114) . The IC50 value is < 0.5 nM, as measured in 100μL reaction mixture containing 1.25 ng trypsin (Sigma, Catalog # T4799), 10 μM substrate, 50 mM Tris, 10 mM CaCl2 , 0.15 M NaCl, 0.05% Brij 35, pH 7.5 .

Met1-Lys289

The secreted recombinant mouse TFPI consists of 272 amino acids and has a calculated molecular mass of 31 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 45 kDa protein in SDS-PAGE under reducing conditions.

Mouse