TFPI2-eiwit (C-His, mens)
Kwaliteit
ISO Gecertificeerd
Levering
24-48 uur
Technische Specificaties
TFPI2
PP5, REF1, TFPI-2
• HEK 293
C-His
< 1.0 EU per μg of the protein as determined by the LAL method
≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK (Dnp) -NH2 (Anaspec, Catalog#27114) . The IC50 value is < 2 nM, as measured in 100μL reaction mixture containing 1.25 ng trypsin (Sigma, Catalog#T4799), 10 μM substrate, 50 mM Tris, 10 mM CaCl2, 0.15M NaCl , 0.05% Brij-35, pH 7.5.
Met1-Lys213
The secreted recombinant humanTFPI2 comprises 202 amino acids and predicts a molecular mass of 23.2 kDa. It migrates as an approximately 32-34 kDa band in SDS-PAGE under reducing conditions.
Human
Beschrijving
TFPI2-eiwit (C-His, mens) Beschikbaar in 100 µg. Bestel eenvoudig online met snelle levering.
Specificaties
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