TIGIT Sigle Domin Antilichaam [2D7]

TIGIT Antibody: The T cell immunoreceptor with Ig and ITIM domains (TIGIT) is a member of the PVR (poliovirus receptor) family of immunoglobin proteins. It is expressed on several classes of T cells including follicular B helper T cells (TFH) . TIGIT has been shown to bind PVR with high affinity; this binding is thought to assist interactions between TFH and dendritic cells to regulate T cell dependent B cell responses (1) . Similar to other immune checkpoint proteins such as PD-1, TIGIT is upregulated on exhausted T cells in chronic viral infections and cancer. Blockade of both TIGIT and PD-1 pathways leads to tumor rejection in mice suggesting that it may be of therapeutic use against cancer (2) .

2016332

Q495A1

NP_776160

Human

TIGIT antibody was raised against a recombinant protein corresponding to amino acids 22-141 of human TIGIT.

2D7

Unconjugated

Primary Antibodies

Immunology

TIGIT Antibody is affinity chromatography purified via Nickel column. Antibody is supplied as a His-tagged purified protein. It also contains a myc-tag for detection.

1 mg/mL

TIGIT Antibody is supplied in PBS.

None

Dry Ice

TIGIT antibody should be stored in working aliquots at -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

SdAb

TIGIT antibody can be used for ELISA starting at 0.1 μ g/mLimmunohistochemistry starting at 10 μ g/mL. For immunofluorescence start at 20 μ g/mL. For flow cytometry at 1 μ g/ml. For immunocytochemistry at 10μ g/mL.

TIGIT

T-cell immunoreceptor with Ig and ITIM domains

Homo sapiens

Stanietsky N, Simic H, Arapovic J, et al. The interaction of TIGIT with PVR and PVRL2 inhibits human NK cell cytotoxicity. Proc Natl Acad Sci USA 2009; 106:17858-63.

Johnston RJ, Comps-Agrar L, Hackney J, et al. The immunoreceptor TIGIT regulates antitumor and antiviral CD8 (+) T cell effector function. Cancer Cell 2014; 26:923-37.

TIGIT Antibody: T-cell immunoreceptor with Ig and ITIM domains, VSIG9, VSTM3, WUCAM

ELISA, IHC-P, ICC, IF, Flow

256600228

Liquid