TIM-1 Antilichaam

TIM-1 Antibody: The human form of TIM-1 was initially discovered as a membrane glycoprotein through which the hepatitis A virus can gain entry into a cell. It was also identified as kidney injury molecule 1 (Kim-1), a predicted adhesion molecule that is upregulated on the surfaces of kidney epithelia. It is also expressed on T helper 2 (Th2) cells of the immune system, and following the binding of its natural ligand TIM-4, stimulates T cell expansion and cytokine production in response to viral challenge. It has been suggested that hyperactivation of TIM-1 leads to an increased level of Th2 responsiveness and asthma susceptibility, and antibodies to TIM-1 may therefore be a novel approach to treating asthma.

26762

Q96D42

NP_036338

Human, Mouse

3809

Unconjugated

Primary Antibodies

Independent Antibody Validation in Cell lines (Figure 2) shows similar TIM-1 expression profile in human cell lines detected by two independent anti-TIM-1 antibodies that recognize different epitopes, 3809 against N-terminus domain and 3811 against the central domain. TIM-1 proteins are detected in all the tested cell lines except MCF-7 at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines3809 3811 siRNA knockdown validation (Figure 3) : Anti-TIM-1 antibody (3809) specificity was further verified by TIM-1 specific siRNA knockdown. TIM-1 signal in HeLa cells transfected with TIM-1 siRNAs was much weaker in comparison with that in HeLa cells transfected with control siRNAs. siRNA knockdown validation3809

Immunology, Neuroscience, Signal Transduction, Growth Factors

TIM-1 Antibody is affinity chromatography purified via peptide column.

Cat. No. 1202 - A431 Cell Lysate

1 mg/mL

Predicted species reactivity based on immunogen sequence: Rat: (56.3%)

TIM-1 Antibody is supplied in PBS containing 0.02% sodium azide.

None

Blue Ice

TIM-1 antibody can be stored at 4˚ C up to one year. Antibodies should not be exposed to prolonged high temperatures.

IgG

Human TIM-1 has 5 isoforms, including isoform a precursor (364aa, 39kD), isoform a (364aa, 39kD), isoform b precursor (401aa, 44kD), isoform b (359aa, 39kD), and isoform c (359aa, 39kD) . This antibody detects human isoform b precursor. Mouse TIM-1 has 3 isoforms, including isoform a precursor (305aa, 33kD), isoform b precursor (282aa, 31kD) and isoform b (282aa, 31kD) . Rat TIM-1 has only one isoform identified so far (307aa, 34kD) .

HAVCR1

Cat. No. 1203 - A549 Cell Lysate

Cat. No. 1224 - Daudi Cell Lysate

Cat. No. 11-401 - Human Uterus Tissue Slide

Hepatitis A virus cellular receptor 1

Homo sapiens

Feigelstock et al. J. Virol. 1998; 72:6621-8

Ichimura et al. J. Biol. Chem. 1998; 273:4135-42

Meyers et al. Mol. Med. 2005; 11:362-9

Mayers et al. Nat. Immunol. 2005;6:455-64.

TIM-1 Antibody: TIM, KIM1, TIM1, HAVCR, KIM-1, TIM-1, TIMD1, TIMD-1, HAVCR-1, Hepatitis A virus cellular receptor 1, Kidney injury molecule 1, HAVcr-1

ELISA, WB, IHC-P, IF

153085427

Liquid