TIM-1 Antilichaam

TIM-1 Antibody: The human form of TIM-1 was initially discovered as a membrane glycoprotein through which the hepatitis A virus can gain entry into a cell. It was also identified as kidney injury molecule 1 (Kim-1), a predicted adhesion molecule that is upregulated on the surfaces of kidney epithelia. It is also expressed on T helper 2 (Th2) cells of the immune system, and following the binding of its natural ligand TIM-4, stimulates T cell expansion and cytokine production in response to viral challenge. It has been suggested that hyperactivation of TIM-1 leads to an increased level of Th2 responsiveness and asthma susceptibility, and antibodies to TIM-1 may therefore be a novel approach to treating asthma.

26762

Q96D42

NP_036338

Human

3811

Unconjugated

Primary Antibodies

Independent Antibody Validation in Cell lines (Figure 2) shows similar TIM-1 expression profile in human cell lines detected by two independent anti-TIM-1 antibodies that recognize different epitopes, 3809 against N-terminus domain and 3811 against the central domain. TIM-1 proteins are detected in all the tested cell lines except MCF-7 at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines3809 3811 siRNA knockdown validation (Figure 3) : Anti-TIM-1 antibody (3811) specificity was further verified by TIM-1 specific siRNA knockdown. TIM-1 signal in HeLa cells transfected with TIM-1 siRNAs was much weaker in comparison with that in HeLa cells transfected with control siRNAs. siRNA knockdown validation3811

Immunology, Neuroscience, Signal Transduction, Growth Factors

TIM-1 Antibody is affinity chromatography purified via peptide column.

Cat. No. 1202 - A431 Cell Lysate

1 mg/mL

Predicted species reactivity based on immunogen sequence: Mouse: (50%), Rat: (43.7%)

TIM-1 Antibody is supplied in PBS containing 0.02% sodium azide.

None

Blue Ice

TIM-1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IgG

Human TIM-1 has 5 isoforms, including isoform a precursor (364aa, 39kD), isoform a (364aa, 39kD), isoform b precursor (401aa, 44kD), isoform b (359aa, 39kD), and isoform c (359aa, 39kD) . This antibody detects human isoform b precursor. Mouse TIM-1 has 3 isoforms, including isoform a precursor (305aa, 33kD), isoform b precursor (282aa, 31kD) and isoform b (282aa, 31kD) . Rat TIM-1 has only one isoform identified so far (307aa, 34kD) .

HAVCR1

Cat. No. 1203 - A549 Cell Lysate

Cat. No. 1224 - Daudi Cell Lysate

Cat. No. 1210 - 293 Cell Lysate

Hepatitis A virus cellular receptor 1

Homo sapiens

Feigelstock et al. J. Virol. 1998; 72:6621-8

Ichimura et al. J. Biol. Chem. 1998; 273:4135-42

Meyers et al. Mol. Med. 2005; 11:362-9

Mayers et al. Nat. Immunol. 2005;6:455-64.

TIM-1 Antibody: TIM, KIM1, TIM1, HAVCR, KIM-1, TIM-1, TIMD1, TIMD-1, HAVCR-1, Hepatitis A virus cellular receptor 1, Kidney injury molecule 1, HAVcr-1

ELISA, WB

153085427

Liquid