TIM3 Antilichaam [1H4]

The immune checkpoint protein TIM3 is a member of the immunoglobulin superfamily and TIM family of proteins that was initially identified as a specific marker of fully differentiated IFN-γ producing CD4 T helper 1 (Th1) and CD8 cytotoxic cells. It is a Th1-specific cell surface protein that regulates macrophage activation and negatively regulates Th1-mediated auto- and alloimmune responses, and is also highly expressed on regulatory T cells, monocytes, macrophages, and dendritic cells (1) . TIM3 and PD-1 are co-expressed on most CD4 and CD8 T cells infiltrating solid tumors or in hematologic malignancy in mice; blocking TIM3 in conjugation with a PD-1 blockade increases the functionality of exhausted T cells and synergizes with to inhibit tumor growth (2,3) .

84868

Q8TDQ0

NP_116171

Human

TIM-3 antibody was raised against the extracellular domain of human TIM-3

1H4

Unconjugated

Primary Antibodies

Cell Cycle, Cancer, Immunology

TIM-3 Antibody is supplied as protein A purified IgG1.

1 mg/mL

TIM-3 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.

None

Blue Ice

TIM-3 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IgG1, k

TIM-3 antibody can be used for ELISA starting at 0.1 μ g/mL. For Immunocytochemistry start at 1 μ g/mL. For Flow Cytometry start at 0.1 μ g/ml.

HAVCR2

Hepatitis A virus cellular receptor 2

Homo sapiens

Monney L, Sabatos CA, Gaglia JL, et al. Th1-specific cell surface protein Tim-3 regulates macrophage activation and severity of autoimmune disease. Nature 2002; 415:536-41.

Sakuishi K, Apetoh L, Sullivan JM, et al. Targeting Tim-3 and PD-1 pathways to reverse T cell exhaustion and restore anti-tumor immunity. J Exp Med 2010; 207:2187–94.

Zhou Q, Munger ME, Veenstra RG, et al. Coexpression of Tim-3 and PD-1 identifies a CD8+ T-cell exhaustion phenotype in mice with disseminated acute myelogenous leukemia. Blood 2011; 117:4501–10.

TIM-3 Antibody: Hepatitis A virus cellular receptor, HAVCR2, TIM3, CD366, KIM-3, TIMD3, TIMD-3

ELISA, ICC, Flow

84868

Liquid