TNF-alfa-ELISA [CE]

TheDiaSource TNF-α -ELISAis a solid phase Enzyme Amplified Sensitivity Immunoassay performed on microtiterplate. The assay uses monoclonal antibodies (MAbs) directed against distinct epitopes of TNF-α. Calibrators and samples react with the capture monoclonal antibody (MAb 1) coated on microtiter well and with a monoclonal antibody (MAb 2) labelled with horseradish peroxidase (HRP). After an incubation period allowing the formation of a sandwich: coated MAb 1 – human TNF-α – MAb 2 – HRP, the mic

HRP

ELISA

Serum

200 µL

7-518 pg/mL

0,7 pg/mL

ELISA

60 Weeks

https://cdn.gentaur.com/products/22/69ae95b01125b5183b569c2e/ifu/kap1751.pdf

4,5 h at RT with shaking 700 rpm

Inflammation Markers

TNF-a

1 pg = 40 mIU of NIBSC IS 87/650

2 levels