TNF-alfa (menselijk) (multimerisch) (Recombinant)

Source :HEK 293 cells Specific Binds to human and mouse TNF-R1 and TNF-R2.Tumor necrosis factor (TNF, cachexin or cachectin and formerly known as tumor necrosis factor-alpha) is a cytokine involved in systemic inflammation and is a member of a group of cytokines that stimulate the acute phase reaction. MultimericTNF-alphaâ„¢ is a high activity construct in which two trimeric TNF-alpha ligands are artificially linked via the collagen domain of ACRP30. The receptor TNF-R1 is activated by both the membrane-bound and soluble trimeric forms of TNF-alpha, whereas the receptor TNF-R2 only responds to the membrane-bound form of TNF-alpha (TNF-alpha (human) (multimeric) (rec.) (32-190048) . Since the MultimericTNF-alphaâ„¢ mimics the membrane-bound form (forms oligomers higher than trimer), it is the only TNF-alpha protein that can activate the TNF-R2. For TNF-R1 activation, either normal" TNF-alpha or MultimericTNF-alphaâ„¢ can be used."

MultimericTNF-alphaâ„¢; ACRP30headless:TNF-alpha; ACRP30headless:TNFSF2; ACRP30headless:Tumor Necrosis Factor-alpha

Human

Functional Assay

>=95% (SDS-PAGE)

Reconstitute with 100?l sterile water.0.1mg/mL after reconstitution. Lyophilized. Contains PBS.

Short Term Storage +4°C ; Long Term Storage-20°C ; After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. PBS containing at least 0. 1% BSA should be used for further dilutions. Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

MW :~34kDa (SDS-PAGE) Biological Activity: Activates human and mouse TNF-R1 and TNF-R2. Induces cell death at a concentration range of 0.01-10ng/ml (ED50= 0.05ng/ml or 2 x 10E7 Units/mg; WEHI 164 cells) . Endotoxin Content <0.02EU/μg purified protein (LAL test; Lonza) .

The extracellular domain of human TNF-alpha (aa 85-233) is fused at the N-terminus to mouse ACRP30headless (aa 18-111) and a FLAG®-tag.