TNF-eiwit (inheems, menselijk)

TNF

DIF, TNF-alpha, TNFA, TNFSF2, TNLG1F

Native

< 1.0 EU per μg protein as determined by the LAL method.

> 90 % as determined by SDS-PAGE.

1.Anti-TNFa (Research Grade Adalim*mab Biosimilar) captured on Protein A chip can bind Amine-Labeled Biotinylated Recombinant Human TNF-alpha Protein (Cat. No. 10602-HNAE-B) with an affinity constant of 0.7767 nM as determined in an SPR assay (Biacore T200) (Routinely tested) . 2.Immobilized Anti-TNFa (Research Grade Adalim*mab Biosimilar) at 5 μg/mL (100 μL/well) can bind Recombinant Human TNF-alpha / TNFA Protein, Biotinylated (Cat: 10602-HNAE-B), the EC50 is 1.3-3.9 ng/mL (Routinely tested) . 3.Immobilized Anti-TNFa (Research Grade Inflix*mab Biosimilar) at 5 μg/mL (100 μL/well) can bind Recombinant Human TNF-alpha / TNFA Protein, Biotinylated (Cat: 10602-HNAE-B), the EC50 is 0.1-1.5 ng/mL (Routinely tested) . 4.Immobilized Anti-TNFa (Research Grade Golim*mab Biosimilar) at 5 μg/mL (100 μL/well) can bind Recombinant Human TNF-alpha / TNFA Protein, Biotinylated (Cat: 10602-HNAE-B), the EC50 is 0.1-1.0 ng/mL (Routinely tested) . 5.Immobilized Recombinant Human TNFRSF1A Protein (Fc Tag) (Cat: 10872-H02H) at 5 μg/mL (100 μL/well) can bind Recombinant Human TNF-alpha / TNFA Protein, Biotinylated (Cat: 10602-HNAE-B), the EC50 is 3.9-11.7 ng/mL (QC tested) . 6.Immobilized Recombinant Human TNFR2 / CD120b / TNFRSF1B Protein (ECD, His & Fc Tag) (Cat: 10417-H03H) at 5 μg/mL (100 μL/well) can bind Recombinant Human TNF-alpha / TNFA Protein, Biotinylated (Cat: 10602-HNAE-B), the EC50 is 2.6-8 ng/mL (Routinely tested) .

Val77-Leu233

The recombinant human TNF consists of 158 amino acids and predicts a molecular mass of 17.5 kDa.

Human