TNFRSF12A / FN14 / TWEAKR eiwit (hFc dag)

Fn14 (tumor necrosis factor receptor superfamily, member 12A), also known as TNFRSF12A, is the receptor for TNFSF12/TWEAK. Fn14 shares 82% amino acid identity with the mouse sequence. It contains a signal peptide, an extracellular domain, a membrane-anchoring domain, and a cytoplasmic domain. In response to FGF1, calf serum, or phorbol ester stimulation of human quiescent fibroblasts in vitro, the level of Fn14 is increased. A 1.2-kb FN14 transcript was expressed at high levels in heart, placenta, and kidney, at intermediate levels in lung, skeletal muscle, and pancreas, and at low levels in brain and liver. Also, elevated FN14 expression was found in human liver cancer cell lines and hepatocellular carcinoma specimens. Expression of mouse Fn14 was upregulated in hepatocellular carcinoma nodules that develop in 2 different transgenic mouse models of hepatocarcinogenesis. TNFRSF12A is the weak inducer of apoptosis in some cell types. It promotes angiogenesis and the proliferation of endothelial cells. TNFRSF12A may modulate cellular adhesion to matrix proteins.

CD266 Protein; FN14 Protein; TWEAKR Protein

Q9NP84-1

Human

Human

HFc-Tag

See Datasheet send with the product

< 1.0 EU per μg of the protein as determined by the LAL method

> 95 % as determined by SDS-PAGE

Immobilized Cynomolgus mFc-TNFSF12 at 10 μg/mL (100 μL/well) can bind human Fc-TNFRSF12A. The ED50 of human Fc-TNFRSF12A is 0.07-0.15 μg/mL.

313 (monomer)

Lyophilized

PBS, pH 7.4

See Datasheet

37 kDa (glycosylated monomer)

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

HEK 293 cells