TNFRSF1B eiwit (C-His, Mouse)

TNFRSF1B

CD120b, p75, TNF-alphaR2, TNF-R-II, TNF-R2, TNF-R75, TNFalpha-R2, TNFBR, Tnfr-1, Tnfr-2, Tnfr2, TNFR80, TNFRII, Tnfrsf1b

C-His

< 1.0 EU per μg of the protein as determined by the LAL method

> 97 % as determined by SDS-PAGE. ≥90 % as determined by SEC-HPLC.

1.Immobilized Recombinant Mouse TNF-alpha / TNFA Protein (ECD) (Cat: 50349-MNAE) at 2 μg/mL (100 μL/well) can bind Recombinant Mouse TNFR2 / CD120b / TNFRSF1B Protein (His Tag) (Cat: 50128-M08H), the EC50 is 1.0-4.0 ng/mL. 2.Measured by its ability to inhibit TNFα-mediated cytotoxicity in L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D.The ED50 for this effect is typically 1-3 μg/mL in the presence of 0.1 ng/mL of recombinant mouse TNFα.

Met1-Gly258

The recombinant mouse TNFRSF1B consists of 247 amino acids and has a predicted molecular mass of 26.8 kDa. In SDS-PAGE under reducing conditions, rm TNFRSF1B migrates as an approximately 45 kDa band due to glycosylation.

Mouse