TNFRSF9 eiwit (C-human IgG1-Fc, muis)

TNFRSF9

4-1BB, A930040I11Rik, Cd137, CDw137, ILA, Ly63, Tnfrsf9

C-human IgG1-Fc

< 1.0 EU per μg of the protein as determined by the LAL method

> 90 % as determined by SDS-PAGE

1.Measured by its binding ability in a functional ELISA. Immobilized mouse His-TNFSF9 (Cat:50067-M07H) at 10 μg/ml (100 μl/well) can bind mouse TNFRSF9-Fc, The EC50 of mouse TNFRSF9-Fc is 12.0-29.0 ng/ml. 2.Loaded Recombinant Mouse 4-1BB/CD137 Protein, hFc Tag (Cat. No. 50811-M02H) on proA Biosensor, can bind Recombinant Mouse 4-1BB Ligand/TNFSF9 Protein, His Tag (Cat. No. 50067-M07H) with an affinity constant of 0.807 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

Met1-Leu211

The secreted recombinant mouse TNFRSF9/Fc is a disulfide-linked homodimer. The reduced monomer comprises 429 amino acids and has a calculated molecular mass of 47 kDa. As a result of glycosylation, the apparent molecular mass of the monomer is approximately 65-75 kDa in SDS-PAGE under reducing conditions.

Mouse