TNFSF11-eiwit (N-cleavage, mens)

TNFSF11

CD254, hRANKL2, ODF, OPGL, OPTB2, RANKL, sOdf, TNLG6B, TRANCE

N-cleavage

<100 EU per mg protein.

≥ 90 % as determined by SDS-PAGE. ≥ 90% as determined by SEC-MALS.

1. Immobilized human TNFSF11 (Cat: 11682-HNCH) at 2 μg/ml (100 μl/well) can bind human Osteoprotegerin hFc (Cat: 10271-H02B), The EC50 of human Osteoprotegerin hFc (Cat: 10271-H02B) is 5-40 ng/ml. 2. The bioactivity of hRANKL was determined by measuring the ability of hRANKL to induce TRAP activity in Raw 264.7 cells. The ED50 for this effect is typically 1.5-7.5 ng/mL.

Gly64-Asp245

The recombinant human TNFSF11 consists of 182 amino acids and has a calculated molecular mass of 20.5 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of the protein is approximately 27 kDa due to the glycosylation.The molecular weight of this protein is around 68.7 kDa verified by SEC-MALS.

Human