TOPBP1 Antilichaam / DNA topo-isosomerase II-bindend eiwit 1

Western Blot: 0.25-0.5 µg/mL, Immunohistochemistry: 2-5 µg/mL, Immunocytochemistry/Immunofluorescence: 5 µg/mL, Immunoprecipitation: 2-4ug/500ug of lysate, ELISA: 0.1-0.5 µg/mL

Q92547

Human, Mouse, Rat

E.coli-derived human TOPBP1 recombinant protein (Position: E217-Q1459) was used as the immunogen for the TOPBP1 antibody.

WB, IHC, ICC/IF, IP, ELISA

Immunogen affinity purified

Lyophilized

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

This TOPBP1 antibody is available for research use only.

After reconstitution, the TOPBP1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Optimal dilution of the TOPBP1 antibody should be determined by the researcher.

Nuclear, cytoplasmic

Immunofluorescent staining of TOPBP1 using anti-TOPBP1 antibody (green) and anti-Beta Tubulin antibody (red) . TOPBP1 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/ml rabbit anti-TOPBP1 antibody and mouse anti-Beta Tubulin antibody overnight at 4oC. DyLight 488 Conjugated Goat Anti-Rabbit IgG and Cy3 Conjugated Goat Anti-Mouse IgG were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. Visualize using a fluorescence microscope and filter sets appropriate for the label used. TOPBP1 displays a punctate nuclear distribution, consistent with its localization to replication and DNA damage–response foci during interphase. The heterogeneous green signal reflects differential concentration within the nucleoplasm, with nucleoli appearing as unstained regions. Beta-tubulin marks the cytoskeleton in red.