TPH2 Antilichaam / Tryptofaanhydroxylase 2

Western blot: 1:500-1:2000, Immunohistochemistry: 1:50-1:200, Immunofluorescence: 1:50-1:200

Q8IWU9

Mouse, Rat

Recombinant protein with human THP2 (Position: M1-I490) was used as the immunogen for the TPH2 antibody.

31T01

WB, IHC, IF

Affinity chromatography

Liquid

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA.

This TPH2 antibody is available for research use only.

Store the TPH2 antibody at -20°C.

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA

Optimal dilution of the TPH2 antibody should be determined by the researcher.

Immunohistochemical staining of TPH2 using anti-TPH2 antibody. TPH2 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution) . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-TPH2 antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.