TRAIL R2 / CD262 / TNFRSF10B eiwit (Zijn label)

Tumor necrosis factor receptor superfamily, member 10b, official symbol TNFRSF10B, also known as Death receptor 5, CD262, TNF-related apoptosis-inducing ligand receptor 2 (TRAIL R2), is a member of the TNF-receptor superfamily, and contains an intracellular death domain. This receptor can be activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL/APO-2L), and transduces an apoptosis signal. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein. TRAIL R2/CD262/TNFRSF10B was purified independently as the only receptor for TRAIL detectable on the surface of two different human cell lines that undergo apoptosis upon stimulation with TRAIL. TRAIL R2/CD262/TNFRSF10B contains two extracellular cysteine-rich repeats, typical for TNF receptor (TNFR) family members, and a cytoplasmic death domain. TRAIL R2/CD262/TNFRSF10B mediates apoptosis via the intracellular adaptor molecule FADD/MORT1. TRAIL receptors can signal both death and gene transcription, functions reminiscent of those of TNFR1 and TRAMP, two other members of the death receptor family. Defects in TRAIL R2/CD262/TNFRSF10B may be a cause of head and neck squamous cell carcinomas (HNSCC) also known as squamous cell carcinoma of the head and neck.

CD262 Protein; DR5 Protein; KILLER Protein; KILLER/DR5 Protein; TRAIL-R2 Protein; TRAILR2 Protein; TRICK2 Protein; TRICK2A Protein; TRICK2B Protein; TRICKB Protein; UNQ160/PRO186 Protein; ZTNFR9 Protein

NP_003833.4

Human

Human

His-Tag

See Datasheet send with the product

< 1.0 EU per μg of the protein as determined by the LAL method

≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.

1. Measured by its binding ability in a functional ELISA. Immobilized human TNFRSF10B at 10 μg/mL (100 μL/well) can bind biotinylated TNFSF10 with a linear range of 0.625-20 ng/mL. 2. Loaded Recombinant Human DR5/TRAIL R2 Protein, His Tag on HIS1K Biosensor, can bind Recombinant Human TRAIL/TNFSF10 Protein with an affinity constant of 0.122 nM as determined in BLI assay (Sartorius Octet RED384) .

138

Lyophilized

PBS, pH 7.4

See Datasheet

22 kDa (glycosylated)

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

HEK 293 cells