TRIM21 Antilichaam

TRIM21, a member of the tripartite motif (TRIM) family, is a part of the RoSSA ribonucleoprotein, which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus (1) . RoSSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus (2) . TRIM21 also acts as a cytosolic antibody receptor; detection of intracellular antibodies activate immune signaling by stimulating the transcription factor pathways of NF-kappaB, AP-1, IRF3, IRF5, and IRF7, resulting in the production of proinflammatory cytokines and the induction of an antiviral state (3) .

6737

P19474

NP_003132

Human

7729

Unconjugated

Primary Antibodies

Independent Antibody Validation in Cell lines (Figure 2) shows similar TRIM21 expression profile in human cell lines detected by two independent anti-TRIM21 antibodies that recognize different epitopes, 7729 against central domain and 18-413 against recombinant fusion protein. TRIM21 proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines7729 18-413

Innate Immunity

TRIM21 antibody is affinity chromatography purified via peptide column.

Cat. No. 1302 - Human Lung Tissue Lysate

1 mg/mL

Predicted species reactivity based on immunogen sequence: Bovine (73%), Mouse (69%) and Rat (69%) .

TRIM21 Antibody is supplied in PBS containing 0.02% sodium azide.

None

Blue Ice

TRIM21 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.

IgG

TRIM21 antibody is human specific. This antibody is predicted to not cross-react with TRIM6.

Human TRIM21 has 2 isoforms, including isoform 1 (475aa, 54kD) and isoform 2 (398aa, 45kD) . Mouse TRIM21 has one isoform (470aa, 54kD) and Rat TRIM21 also has one isoform (471aa, 54kD) . 7729 can detect human and possibly mouse and rat.

TRIM21

Cat. No. 11-801 - Human Small Intestine Tissue Slide

Cat. No. 1201 - HeLa Cell Lysate

Cat. No. 1220 - SK-N-SH Cell Lysate

Tripartite motif containing 21

Homo sapiens

Oke and Wahren-Herlenius. J. Autoimmun. 2012; 39:77-82.

Frank et al. Biochem. J. 1995; 305:359-62.

McEwan. Nat. Immunol. 14:327-36.

TRIM21 Antibody: SSA, RO52, SSA1, RNF81, Ro/SSA, E3 ubiquitin-protein ligase TRIM21, 52 kDa Ro protein, SS-A

ELISA, WB, IHC-P, IF

15208660

Liquid