TruNGS® BRCA Plus Kit (Inclusief Bioinformatica workflow en rapportage)

This product is a complete NGS solution to detect germline as well as somatic variants (SNVs and Indels) in BRCA1, BRCA2 and STK11 genes from nucleic acid extracted from blood/fresh frozen/formalin fixed paraffin embedded (FFPE) tissue. The kit has been designed to detect SNVs and Indels in BRCA1, BRCA2 and STK11 genes from nucleic acid extracted from blood/fresh frozen/formalin fixed paraffin embedded (FFPE) tissue specimens. The protocol involves two major steps: (A) Library preparation (input DNA fragmentation, adapters ligation, PCR amplification of individual libraries) ; and (B) Hybrid Capture and target enrichment (library pooling, probes hybridization, capture, and post- capture PCR amplification) For use on Illumina and MGI platforms only RUO (Research Use Only) An example of the report can be found here