TRUPCR® JAK-2 Allele Burden Kit

• CE-IVD • Our JAK-2 V617F Allele Burden Kit allows highly sensitive quantification, up to 1% mutant sequence copies in a background of 99% wild-type DNA. • High specificity due to site-specific hydrolysis probes utilized in the reaction. • Quantitative standards are calibrated to WHO 1st International Reference Panel for Genomic JAK2 V617F mutation. • Easy workflow & compatible with most of the Real-time PCR equipment. • Available in different pack sizes.

The TRUPCR® JAK-2 V617F Allele Burden Kit is a Real-time polymerase chain reaction (PCR) assay for the quantification of V617F mutation in JAK-2 (Janus Kinase 2) gene against a background of wild-type genomic DNA. This mutation is a guanine to thymidine transversion in position 1849 of the JAK-2 gene, which leads to a valine to phenylalanine substitution in position 617 of the protein (V617F) . The test utilizes mutation-specific primers and a JAK-2 V617F/G1849T targeted fluorescent probe to detect low-copy number (1%) JAK-2 V617F-mutant DNA in cancer tissue. The TRUPCR JAK-2 V617F Mutation Detection Kit is a highly selective and sensitive assay for quantification of V617F mutation in the JAK-2 oncogene.