TTC36 Antilichaam / Tetratricopeptide herhaalde proteïne 36

Western Blot: 0.25-0.5 µg/mL, Immunocytochemistry: 5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

A6NLP5

Human, Mouse

E.coli-derived human TTC36 recombinant protein (Position: M1-D187) was used as the immunogen for the TTC36 antibody.

WB, ICC, IF, FACS, ELISA

Immunogen affinity purified

Lyophilized

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

This TTC36 antibody is available for research use only.

After reconstitution, the TTC36 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Optimal dilution of the TTC36 antibody should be determined by the researcher.

Nuclear

Immunofluorescent staining of TTC36 using anti-TTC36 antibody (green) and anti-Beta Tubulin antibody (red) . TTC36 was detected in immunocytochemical section of cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/ml rabbit anti-TTC36 antibody and mouse anti-Beta Tubulin antibody overnight at 4oC. DyLight 488 Conjugated Goat Anti-Rabbit IgG and Cy3 Conjugated Goat Anti-Mouse IgG were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. Visualize using a fluorescence microscope and filter sets appropriate for the label used. TTC36 antibody (green) shows nuclear-enriched staining with nucleolar sparing, consistent with reported nuclear localization. Beta-tubulin antibody (red) marks the cytoplasmic microtubule network surrounding the nucleus.