TYMP Antilichaam/Thymidinefosforylase / PD-ECGF

Western Blot: 0.25-0.5 µg/mL, Immunoprecipitation: 2-4ug/500ug of lysate, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

P19971

Human

E.coli-derived human PD-ECGF/TYMP recombinant protein (Position: P13-Q482) was used as the immunogen for the TYMP antibody.

WB, IP, FACS, ELISA

Immunogen affinity purified

Lyophilized

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

This TYMP antibody is available for research use only.

After reconstitution, the TYMP antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Optimal dilution of the TYMP antibody should be determined by the researcher.

Immunoprecipitating PD-ECGF/TYMP in SiHa whole cell lysate. Western blot analysis of PD-ECGF/TYMP using anti-TYMP antibody. Lane 1: SiHa whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-TYMP antibody in SiHa whole cell lysate, Lane 3: anti-TYMP antibody (2ug) + SiHa whole cell lysate (500ug) . After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TYMP antibody at a dilution of 0.5 ug/ml and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. The input lane shows the characteristic doublet at approximately 50 kDa and in the high 40 kDa region. In contrast, the immunoprecipitated material displays a single band at approximately 50 kDa, indicating selective enrichment of the full length TYMP species. The control IgG lane shows no detectable signal, confirming specificity of the immunoprecipitation.