Tyrosine Hydroxylase Antilichaam

Tyrosine Hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines Dopamine and Norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons.

25085

P04177

P04177

Human, Mouse, Rat, Dog, Bovine

TH polyclonal antibody was raised against native rat tyrosine hydroxylase, purified from pheochromocytoma.

Unconjugated

Primary Antibodies

Neuroscience, Signal Transduction

Affinity Purified

Batch dependent

None

Blue Ice

For long term storage –80˚ C is recommended, but shorter term storage at –20˚ C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.

60

Tyrosine Hydroxylase antibody is specific for the 60k rat TH in lysates of PC-12 Cells. Applications include Immunofluorescence (IF), Immunohistochemistry (IHC) and Western Blot (WB) . The antibody has been characterized in blot immunolabeling and immunocytochemical studies and cross-reacts with many different mammalian and some non-mammalian forms of TH. When internally tested under ideal conditions the working dilutions were 1:1000 for IHC, IF and WB.

Th

Tyrosine hydroxylase

Rattus norvegicus

Xu, Z.-Q., Lew, J. Y., Harada, K., Aman, K., Goldstein, M., Deutch, A., Haycock, J. W., and Hokfelt, T., "Immunohistochemical studies on phosphorylation of tyrosine hydroxylase in central catecholamine neurons using site- and phosphorylation state-specific antibodies, " Neuroscience, 82 (1998) 727-738.

Haycock, J.W. "Multiple forms of tyrosine hydroxylase in human neuroblastoma cells: Quantitation with isoform-specific antibodies, " J. Neurochem. 60 (1993) 493-502.

Haycock, J.W., "Four forms of tyrosine hydroxylase are present in human adrenal medulla, " J. Neurochem. 56 (1991) 2139 - 2142.

Haycock, J.W., "Quantitation of tyrosine hydroxylase protein levels: Spot immunolabeling with an affinity-purified antibody, " Anal. Biochem. 181 (1989) 259 - 266.

WB, IF, IHC

136577

Liquid