TYW5 cDNA

TYW5 (TRNA-YW Synthesizing Protein 5) acts as a component of the wybutosine biosynthesis pathway. It catalyzes the hydroxylation of 7- (a-amino-a-carboxypropyl) wyosine (yW-72) into undermodified hydroxywybutosine (OHyW) .

C2orf60, hTYW5

2q33.1

Human

PATGen (puc19-derived cloning vector)

ATGGCCGGGCAGCACCTCCCGGTACCCCGGCTGGAGGGCGTTTCTCGGGAGCAGTTCATGCAGCACCTCTACCCACAGAGAAAACCTCTTGTGTTGGAAGGGATTGATTTGGGGCCATGTACAAGCAAATGGACAGTGGATTACCTAAGCCAAGTTGGAGGGAAGAAAGAAGTAAAGATTCATGTTGCTGCAGTTGCACAGATGGACTTCATTAGTAAGAACTTTGTATATAGAACTTTACCTTTTGACCAGTTGGTCCAGAGGGCAGCTGAAGAGAAACATAAAGAATTCTTTGTTTCAGAGGATGAGAAATACTACTTACGGTCACTTGGAGAAGACCCTAGAAAGGATGTTGCAGATATCAGAAAGCAGTTTCCTTTGTTGAAAGGAGATATTAAGTTTCCAGAATTCTTCAAAGAGGAACAGTTCTTTTCCAGTGTTTTTCGAATTAGTTCACCAGGATTACAACTATGGACTCATTATGATGTAATGGATAATTTGTTAATACAAGTGACAGGAAAAAAGCGTGTTGTACTCTTCAGTCCTCGAGATGCCCAGTATTTATATTTAAAAGGTACTAAATCAGAAGTACTGAATATAGATAACCCAGACTTGGCTAAATATCCACTTTTTTCCAAGGCTAGAAGATATGAATGTTCCCTTGAAGCTGGTGATGTATTATTCATTCCTGCTTTATGGTTCCATAATGTAATTTCTGAAGAGTTTGGAGTGGGAGTGAATATCTTTTGGAAGCACCTTCCATCTGAATGCTATGATAAAACAGATACCTATGGAAACAAAGATCCTACAGCAGCATCAAGAGCTGCACAAATTCTGGACAGAGCCTTGAAAACACTGGCCGAGTTACCAGAGGAATATAGGGACTTCTATGCACGACGAATGGTCCTACACATTCAAGACAAAGCCTACAGCAAGAACTCTGAGTAA

TYW5, C2orf60, hTYW5, TYW5, ATGD0356-10 µg, ATGD0356-20 µg, ATGD0356-50 µg, ATGD0356-100 µg, ATGD0356-250 µg, ATGD0356-500 µg, ATGD0356-1 mg, ATGD0356-10, ATGD0356-20, ATGD0356-50, ATGD0356-100, ATGD0356-250, ATGD0356-500, ATGD0356-1

Store the plasmid at -20C.

Lyophilized

Epigenomics (Transcription & Translation)

NP_001034782.1

Human

1. Centrifuge at 7000rpm for 1 minute.; ;2. Carefully open the vial and add 100 µL of sterile water to dissolve the DNA.; ; Each tube contains approximately 10 µg of lyophilized plasmid.

NM_001039693.2

939bp

This shuttle vector contains the complete ORF. It is inseted BamH I to Hind III. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.