UAP56 (DDX39B) Mouse Monoclonal Antibody [Kloon ID: LBI8B8]

Splice factor that is required for the first ATP-dependent step in spliceosome assembly and for the interaction of U2 snRNP with the branchpoint. Has both RNA-stimulated ATP binding/hydrolysis activity and ATP-dependent RNA unwinding activity. Even with the stimulation of RNA, the ATPase activity is weak. Can only hydrolyze ATP but not other NTPs. The RNA stimulation of ATPase activity does not have a strong preference for the sequence and length of the RNA. However, ssRNA stimulates the ATPase activity much more strongly than dsRNA. Can unwind 5' or 3' overhangs or blunt end RNA duplexes in vitro. The ATPase and helicase activities are not influenced by U2AF2; the effect of ALYREF/THOC4 is reported conflictingly with [PubMed:23299939] reporting a stimulatory effect. [UniProtKB/Swiss-Prot Function]

Carrier-free (BSA/glycerol-free) DDX39B mouse monoclonal antibody, clone LBI8B8

BAT1; D6S81E; UAP56

Homo sapiens DExD-box helicase 39B (DDX39B), transcript variant 1, mRNA.

7919

Q13838

Unconjugated

Spliceosome

Spliceosome

WB 1:500, IHC 1:500

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Stable for 1 year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. Aliquot will be stable at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

DDX39B

https://www.ncbi.nlm.nih.gov/gene/?term=7919

https://www.uniprot.org/uniprot/Q13838