UBA3 Antilichaam / Ubiquitin-activerend enzym 3 / UBE1C

NEDD8-activating enzyme E1 catalytic subunit (UBA3) is the catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Down-regulates steroid receptor activity. Necessary for cell cycle progression. [UniProt]

Western blot: 1:500-1:2000

Q8TBC4

Human

A synthetic peptide from human NEDD8-activating enzyme E1 catalytic subunit protein was used as the immunogen for the UBA3 antibody.

28U91

WB

Affinity chromatography

Purified

This UBA3 antibody is available for research use only.

Store the UBA3 antibody at -20°C.

Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5 mg/mL BSA

Optimal dilution of the UBA3 antibody should be determined by the researcher.

Western blot testing of human HeLa cell lysate with UBA3 antibody. Predicted molecular weight ~52 kDa.