UBE2N cDNA

UBE2N, also known as uBC13, is a member of the E2 ubiquitin-conjugating enzyme family. It catalyzes the ATP-dependent synthesis of non-canonical polyubiquitin chains, a process that does not lead to proteasomal degradation. It mediates the transcription of several target genes and is thought to play a role in cell cycle progression; cellular differentiation and DNA repair mechanisms that ensure cell survival after DNA damage.

Ubiquitin-conjugating enzyme E2N, UBC13, UbcH-ben

12q22

Human

PATGen (puc19-derived cloning vector)

ATGGCCGGGC TGCCCCGCAG GATCATCAAG GAAACCCAGC GTTTGCTGGC AGAACCAGTT CCTGGCATCA AAGCCGAACC AGATGAGAGC AACGCCCGTT ATTTTCATGT GGTCATTGCT GGCCCTCAGG ATTCCCCCTT TGAGGGAGGG ACTTTTAAAC TTGAACTATT CCTTCCAGAA GAATACCCAA TGGCAGCCCC TAAAGTACGT TTCATGACCA AAATTTATCA TCCTAATGTA GACAAGTTGG GAAGAATATG TTTAGATATT TTGAAAGATA AGTGGTCCCC AGCACTGCAG ATCCGCACAG TTCTGCTATC GATCCAGGCC TTGTTAAGTG CTCCCAATCC AGATGATCCA TTAGCAAATG ATGTAGCGGA GCAGTGGAAG ACCAACGAAG CCCAAGCCAT AGAAACAGCT AGAGCATGGA CTAGGCTATA TGCCATGAAT AATATTTAA

UBE2N, HEL-S-71, UBC13, UbcH-ben, UbcH13, UBCHBEN, UBE2N, ATGD0044-10 µg, ATGD0044-20 µg, ATGD0044-50 µg, ATGD0044-100 µg, ATGD0044-250 µg, ATGD0044-500 µg, ATGD0044-1 mg, ATGD0044-10, ATGD0044-20, ATGD0044-50, ATGD0044-100, ATGD0044-250, ATGD0044-500, ATGD0044-1

Store the plasmid at -20C.

Lyophilized

Ubiquitination

603679

NP_003339.1

Human

1. Centrifuge at 7000rpm for 1 minute.; ;2. Carefully open the vial and add 100 µL of sterile water to dissolve the DNA.; ; Each tube contains approximately 10 µg of lyophilized plasmid.

NM_003348.3

459bp

This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.