UBE2T Antilichaam / Ubiquitin-conjugerend enzym E2 T / HSPC150

Western Blot: 0.25-0.5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL, Immunofluorescence: 5 µg/mL

Q9NPD8

Human, Mouse, Rat

E.coli-derived human UBE2T recombinant protein (Position: R9-Q145) was used as the immunogen for the UBE2T antibody.

WB, FACS, IF, ELISA

Immunogen affinity purified

Lyophilized

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

This UBE2T antibody is available for research use only.

After reconstitution, the UBE2T antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Optimal dilution of the UBE2T antibody should be determined by the researcher.

IF analysis of UBE2T using anti-UBE2T antibody (green) . UBE2T was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1:100 rabbit anti-UBE2T antibody overnight at 4oC. DyLight 488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. Visualize using a fluorescence microscope and filter sets appropriate for the label used.