UBE3A Antilichaam - middelste regio : HRP (ARP42951_P050-HRP)

Ubiquitin protein ligase E3A

AS, ANCR, E6-AP, HPVE6A, EPVE6AP

7337

Q05086

NP_000453

Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish

The immunogen is a synthetic peptide directed towards the middle region of human UBE3A

UBE3A is an E3 ubiquitin-protein ligase, part of the ubiquitin protein degradation system. This imprinted gene is maternally expressed in brain and biallelically expressed in other tissues. Maternally inherited deletion of this gene causes Angelman Syndrome, characterized by severe motor and intellectual retardation, ataxia, hypotonia, epilepsy, absence of speech, and characteristic facies. The protein also interacts with the E6 protein of human papillomavirus types 16 and 18, resulting in ubiquitination and proteolysis of tumor protein p53.Western blots using two different antibodies against two unique regions of this protein target confirm the same apparent molecular weight in our tests.This gene encodes an E3 ubiquitin-protein ligase, part of the ubiquitin protein degradation system. This imprinted gene is maternally expressed in brain and biallelically expressed in other tissues. Maternally inherited deletion of this gene causes Angelman Syndrome, characterized by severe motor and intellectual retardation, ataxia, hypotonia, epilepsy, absence of speech, and characteristic facies. The protein also interacts with the E6 protein of human papillomavirus types 16 and 18, resulting in ubiquitination and proteolysis of tumor protein p53. Alternative splicing of this gene results in three transcript variants encoding three isoforms with different N-termini. Additional transcript variants have been described, but their full length nature has not been determined.

KLHL38; UBE2D1; UBB; TP53; TAT; AFG3L1P; ATG9A; ASAP3; AHSP; UBE3A; UBC; MEOX2; MAGEA8; JADE1; YOD1; UBE2L3; UBE2D3; PSMD14; SHFM1; ARNTL; LPP; HSF1; DR1; BTF3L4; LRSAM1; P3H1; NPLOC4; IPO11; DBNL; LSM1; ARPC1B; METTL1; SACS; ARC; RAD23A; PTPN3; CEBPA; SC

HRP: Horseradish Peroxidase

Polyclonal Antibody

WB

Affinity Purified

UBE3A is strongly supported by BioGPS gene expression data to be expressed in HeLa

0.5 mg/ml

Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 86%

Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.

All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.

101kDa

Bedard, K.M., (2008) J. Virol. 82 (8), 3894-3902

Wet Ice

875

UBE3A

UBE3A

Rabbit

Ubiquitin-protein ligase E3A

NM_000462

AKNGPDTERLPTSHTCFNVLLLPEYSSKEKLKERLLKAITYAKGFGML