URM1 cDNA

URM1 is ubiquitin-related modifier 1 homolog protein that primarily functions in the post-translational modification of proteins by way of the urmylation pathway. In studies with Saccharomyces cerevisiae, it has been found that urm1 covalently binds to its E1 activating enzyme, uba4p, to conjugate alkyl hydroperoxide reductase (Ahp1) . It is hypothesized that this complex may then play a role in the oxidative-stress response in mammals.

C9orf74

9q34.11

Human

PATGen (puc19-derived cloning vector)

ATGGCTGCGC CCTTGTCAGT GGAGGTGGAG TTCGGAGGTG GTGCGGAGCT CCTGTTTGAC GGTATTAAGA AACATCGAGT CACTTTGCCT GGACAGGAGG AACCCTGGGA CATCCGGAAC CTGCTCATCT GGATCAAGAA GAATTTGCTA AAAGAGCGGC CAGAGTTGTT CATCCAGGGA GACAGCGTGC GGCCAGGAAT TCTGGTGCTG ATTAACGATG CCGACTGGGA GCTACTGGGT GAGCTGGACT ACCAGCTTCA GGACCAGGAC AGCGTCCTCT TCATCTCCAC TCTGCACGGC GGCTGA

URM1, C9orf74, URM1, ATGD0093-10 µg, ATGD0093-20 µg, ATGD0093-50 µg, ATGD0093-100 µg, ATGD0093-250 µg, ATGD0093-500 µg, ATGD0093-1 mg, ATGD0093-10, ATGD0093-20, ATGD0093-50, ATGD0093-100, ATGD0093-250, ATGD0093-500, ATGD0093-1

Store the plasmid at -20C.

Lyophilized

Ubiquitination

612693

NP_112176.1

Human

1. Centrifuge at 7000rpm for 1 minute.; ;2. Carefully open the vial and add 100 µL of sterile water to dissolve the DNA.; ; Each tube contains approximately 10 µg of lyophilized plasmid.

NM_030914.3

306bp

This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.