VEGFA Antilichaam / Vasculaire Endotheliale Groeifactor A

This MAb recognizes proteins of 19-22kDa (reducing) and 38kDa-44kDa (non-reducing), identified as various isoforms of Vascular Endothelial Growth Factor or Vascular Permeability Factor (VEGF/VPF) . It is highly specific to VEGF, which is a homodimeric, disulfide-linked glycoprotein with a close homology to platelet derived growth factor (PDGF) . There are multiple isoforms of VEGF containing 206-, 189-, 165-, and 121-amino acid residues. The smaller two isoforms, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two remain cell associated. VEGF/VPF plays an important role in angiogenesis, which promotes tumor progression and metastasis.

Immunohistochemistry (FFPE) : 1-2 µg/mL

P15692

Human

A portion of amino acids 1-200 from human VEGF protein was used as the immunogen for the VEGFA antibody.

VEGF/9366

IHC-P

Protein G affinity

Purified

This VEGFA antibody is available for research use only.

Aliquot the VEGFA antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Optimal dilution of the VEGFA antibody should be determined by the researcher.

Cytoplasmic, cell surface and extracellular (secreted)

IHC staining of FFPE human placenta tissue with VEGFA antibody (clone VEGF/9366) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.