VEGFA (NM 001025366) Humaan Recombinant eiwit

This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulfide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. This gene is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Elevated levels of this protein are found in patients with POEMS syndrome, also known as Crow-Fukase syndrome. Allelic variants of this gene have been associated with microvascular complications of diabetes 1 (MVCD1) and atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been described. There is also evidence for alternative translation initiation from upstream non-AUG (CUG) codons resulting in additional isoforms. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is antiangiogenic. Expression of some isoforms derived from the AUG start codon is regulated by a small upstream open reading frame, which is located within an internal ribosome entry site. The levels of VEGF are increased during infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), thus promoting inflammation by facilitating recruitment of inflammatory cells, and by increasing the level of angiopoietin II (Ang II), one of two products of the SARS-CoV-2 binding target, angiotensin-converting enzyme 2 (ACE2) . In turn, Ang II facilitates the elevation of VEGF, thus forming a vicious cycle in the release of inflammatory cytokines. [provided by RefSeq, Jun 2020]

Recombinant protein of human Vascular endothelial growth factor A (VEGF) produced in Pichia Pastoris.

MVCD1; VEGF; VPF

7422

P15692

NM_001025366

6p21.1

Pichia

Tag Free

Bladder cancer, Cytokine-cytokine receptor interaction, Focal adhesion, mTOR signaling pathway, Pancreatic cancer, Pathways in cancer, Renal cell carcinoma, VEGF signaling pathway

Bladder cancer, Cytokine-cytokine receptor interaction, Focal adhesion, mTOR signaling pathway, Pancreatic cancer, Pathways in cancer, Renal cell carcinoma, VEGF signaling pathway

Resuspend the protein to the desired concentration in proper buffer.

< 0.1 EU per 1 µg of the protein by the LAL

>95% as determined by SDS-PAGE and Coomassie blue staining

Measured in a cell proliferation assay using HUVEC Cells. The ED50 for this effect is typically 1.1 - 1.6 ng/ml.

Lyophilized

Lyophilized from a sterile solution containing 20 mM phosphate buffer, pH7.2

Druggable Genome, Secreted Protein

19.2 kDa

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

VEGFA

Human

NP_001020537

https://www.ncbi.nlm.nih.gov/gene/7422

https://www.uniprot.org/uniprot/P15692

https://www.ncbi.nlm.nih.gov/nuccore/NM_001025366

https://www.ncbi.nlm.nih.gov/nuccore/NP_001020537