Vetvrij antilichaam

Fat Free Antibody: Fat Free, also known as ANG2, was identified though a yeast two-hybrid screen using VP53. It is thought to be a structural and functional homolog of the yeast protein VPS51, a protein that together with VPS52, VPS53, and VPS54 form the Golgi-associated retrograde protein (GARP) complex that mediates the tethering and fusion of endosome-derived transport carriers to the trans-Golgi network (TGN) . Similar to depletion of any of the VPS52, VPS53, or VPS54, depletion of Fat Free using RNAi impairs protein retrieval to the TGN indicating that Fat Free is missing component of the GARP complex in most eukaryotes.

738

Q9UID3

NP_037397

Human, Mouse, Rat

Unconjugated

Primary Antibodies

Homeostasis

Fat Free Antibody is affinity chromatography purified via peptide column.

Cat. No. 1403 - Mouse Brain Tissue Lysate

1 mg/mL

Predicted species reactivity based on immunogen sequence: Bovine: (100%), Chicken: (82%)

Fat Free Antibody is supplied in PBS containing 0.02% sodium azide.

None

Blue Ice

Fat Free antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

86 kDa

IgG

At least two alternatively spliced isoforms are known to exist; this antibody will detect both isoforms.

C11orf2

Chromosome 11 open reading frame 2

Homo sapiens

Perez-Victoria FJ, Schindler C, Magadan JG, et al. Ang2/Fat Free is a conserved subunit of the Golgi-associated retrograde protein complex. Mol. Biol. Cell 2010; 21:3386-95

Ho SY, Lorent K, Pack M, et al. Zebrafish Fat Free is required for intestinal lipid absorption and Golgi apparatus structure. Cell Metab. 2006; 3:289-300.

Perez-Victoria FJ, Mardones GA, and Bonifacino JS. Requirement of the human GARP complex for mannose 6-phosphate-receptor-dependent sorting of cathepsin D to lysosomes. Mol. Biol. Cell 2008; 19:2350-62

Stafford RL, Ear J, Knight MJ, et al. The molecular basis of the Caskin1 and Mint1 interaction with CASK. J. Mol. Biol. 2011; 412:3-13.

Fat Free Antibody: FFR, ANG2, ANG3, C11orf2, C11orf3, FFR, PP5382, Vacuolar protein sorting-associated protein 51 homolog, Another new gene 2 protein

ELISA, WB

8393009

Liquid