Vinculine-antilichaam

Vinculin is a cytoskeletal protein that plays an important role in the regulation of focal adhesions and embryonic development (1) . Three structural vinculin domains include an amino-terminal head, a short flexible proline-rich region and a carboxy-terminal tail (2) . Expression of vinculin were shown to be affected by the level of actin expression (2,3) . Vinculin deficiencies are associated with a decrease in cell adhesion and an increase in cell motility, suggesting a possible role in metastatic growth (4) . Defects in VCL are the cause of cardiomyopathy dilated type 1W (CMD1W) (5) .

7414

P18206

NP_003364

Human, Mouse, Rat

7809

Unconjugated

Primary Antibodies

Independent Antibody Validation in Cell lines (Figure 2) shows similar Vinculin expression profile in human, mouse and rat cell lines detected by three independent anti-Vinculin antibodies that recognize different epitopes, 7489 against C-terminus domain, 7807 against N-terminus domain and 7809 against central domain. Vinculin proteins are detected in the most tested cell lines at different expression levels by the three independent antibodies. Independent Antibody Validation in Cell lines7489 78077809

Homeostasis

Vinculin antibody is affinity chromatography purified via peptide column.

Cat. No. 1202 - A431 Cell Lysate

1 mg/mL

Predicted species reactivity based on immunogen sequence: Pig: (100%)

Vinculin antibody is supplied in PBS containing 0.02% sodium azide.

None

Blue Ice

Vinculin antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.

IgG

Vinculin antibody is human, mouse and rat reactive. At least three isoforms of Vinculin are known to exist.

Human Vinculin has 3 isoforms, including isoform 2 (1134aa, 124kD), isoform 1 (1066aa, 117kD) and isoform 3 (222aa, 25kD) . Mouse Vinculin has one isoform (1066aa, 117kD) and Rat Vinculin also has one isoform (1066aa, 117kD) . 7809 can detect human, mouse and rat.

VCL

Cat. No. 1201 - HeLa Cell Lysate

Cat. No. 1282 - 3T3/NIH Cell Lysate

Vinculin

Homo sapiens

Burridge et al. Annu. Rev. Cell Biol.1988; 4:487-525.

Gilmore et al. J. Cell Sci. 1992; 103:719-31.

Deakin et al. PLoS One 2012; 7:e37990.

Goldmann et al. Cell Biol. Int. 2013; Feb 1.

26873631

Vinculin Antibody: MV, MVCL, CMD1W, CMH15, HEL114, Vinculin, Metavinculin, MV

ELISA, WB, ICC, IF

4507877

Liquid