WEE1 (fosfo Ser642) Antilichaam

Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.

7465

P30291

NP_003381.1

Human, Mouse, Rat

WEE1 (phospho Ser642) antibody was raised against a peptide sequence around phosphorylation site of Serine 642 (S-V-S (p) -L-T) derived from Human WEE1.

Unconjugated

Primary Antibodies

Phospho-Specific

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

1 mg/mL

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Phospho-Specific

Blue Ice

Store antibody at -20˚ C for up to one year.

100 kDa

IgG

The antibody detects endogenous levels of WEE1 only when phosphorylated at serine 642.

Western Blot: 1:500~1:1000

WEE1

WEE1 G2 checkpoint kinase

Homo sapiens

Watanabe N., EMBO J. 14:1878-1891 (1995) .

Cichutek A., Cytogenet. Cell Genet. 93:277-283 (2001) .

Igarashi M., Nature 353:80-83 (1991)

WEE1A, WEE1hu, kinase WEE1

WB

4507917

Liquid