WIBG cDNA

WIBG has been identified as an interacting partner of Mago-Y14. The Mago-Y14 heterodimer is a core component of the EJC (exon junction complex) that is deposited on mRNAs as a consequence of splicing and influences postsplicing mRNA metabolism. This protein is a cytoplasmic RNA-binding protein that is excluded from the nucleus by Crm1. It interacts directly with Mago-Y14 by means of its N-terminal domain.

PYM

12q13.2

Human

PATGen (puc19-derived cloning vector)

ATGGAAGCTG CCGGCAGCCC TGCGGCTACG GAGACAGGCA AGTATATCGC GTCAACACAG CGACCTGACG GGACCTGGCG CAAGCAGCGG AGGGTGAAAG AAGGATATGT GCCCCAGGAG GAGGTCCCAG TATATGAAAA CAAGTATGTG AAGTTTTTCA AGAGTAAACC AGAGTTGCCC CCAGGGCTAA GCCCTGAGGC CACTGCTCCT GTCACCCCAT CCAGGCCTGA AGGTGGTGAA CCAGGCCTCT CCAAGACAGC CAAACGTAAC CTGAAGCGAA AGGAGAAGAG GCGGCAGCAG CAAGAGAAAG GAGAGGCAGA GGCCTTGAGC AGGACTCTTG ATAAGGTGTC CCTGGAAGAG ACAGCCCAAC TCCCCAGTGC TCCACAGGGC TCTCGGGCAG CCCCCACAGC TGCATCTGAC CAGCCTGACT CAGCTGCCAC CACTGAGAAA GCCAAGAAGA TAAAGAACCT AAAGAAGAAA CTCCGGCAGG TGGAAGAGCT GCAGCAGCGG ATCCAGGCTG GGGAAGTCAG CCAGCCCAGC AAAGAGCAGC TAGAAAAGCT AGCAAGGAGG AGGGCGCTAG AAGAGGAGTT AGAGGACTTG GAGTTAGGCC TCTGA

WIBG, PYM, WIBG, ATGD0023-10 µg, ATGD0023-20 µg, ATGD0023-50 µg, ATGD0023-100 µg, ATGD0023-250 µg, ATGD0023-500 µg, ATGD0023-1 mg, ATGD0023-10, ATGD0023-20, ATGD0023-50, ATGD0023-100, ATGD0023-250, ATGD0023-500, ATGD0023-1

Store the plasmid at -20C.

Lyophilized

Epigenomics (Transcription & Translation)

NP_115721.1

Human

1. Centrifuge at 7000rpm for 1 minute.; ;2. Carefully open the vial and add 100 µL of sterile water to dissolve the DNA.; ; Each tube contains approximately 10 µg of lyophilized plasmid.

NM_032345.2

615bp

This shuttle vector contains the complete ORF. It is inseted Nde I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.