WiDr Lysate

WiDr cells are established from a 78 year old female with colon adenocarcinoma. The cells are negative for colon antigen 3 expression and positive for keratin by immunoperoxidase staining. WiDr cells expressed p53 antigen. Growth of WiDR cells is inhibited by tumor necrosis factor alpha (TNF-alpha) . Inhibitors of dihydrofolate reductase are highly cytotoxic to WiDR cells.

Human

WB

2 mg/mL (determined by BCA assay)

WiDr, ATGL0020-10 µg, ATGL0020-20 µg, ATGL0020-50 µg, ATGL0020-100 µg, ATGL0020-250 µg, ATGL0020-500 µg, ATGL0020-1 mg, ATGL0020-10, ATGL0020-20, ATGL0020-50, ATGL0020-100, ATGL0020-250, ATGL0020-500, ATGL0020-1

Aliquot the lysate into single tube and then storing them -80C or below. Avoid repeated freezing and thawing cycles.

Liquid in. SDS-PAGE Sample Buffer (0.12M Tris-HCl buffer pH6.8, 0.4% SDS, 5% glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)

Lysates

Human

All lanes : WiDr Whole Cell Lysate (40ug) ; ; ; ; Lane 1. : Anti-GAPDH antibody (ATGA0181) ; ; Lane 2. : Anti-Beta tubulin antibody (ATGA0196) ;

Heat lysate to 95°C for 5 minutes and rapidly cooling before use.

Western Blot analyzed by both GAPDH and Beta tubulin antibody