XBP-1 Antilichaam

XBP-1 Antibody: X box binding protein 1 (XBP-1) is a key protein in the mammalian unfolded protein response (UPR) that protects the cell against the stress of malfolded proteins in the endoplasmic reticulum (ER) . Upon sensing unfolded proteins, an ER transmembrane endonuclease and kinase termed IRE1p is activated and excises an intron from XBP-1 mRNA. The spliced XBP-1 mRNA results in a 371 amino acid protein (XBP-1s) which is then translocated to the nucleus where it binds to the regulatory elements of downstream genes. Together with other UPR transcription factors such as ATF6, XBP-1 stimulates the production of ER stress proteins including the ER resident protein chaperones glucose regulated protein (GRP) 78 and GRP94.

7494

P17861

P17861

Human, Mouse, Rat

3687

Unconjugated

Primary Antibodies

KO Validation (Figure 2) : XBP-1 expression detected by anti-XBP-1 antibodies was observed in wild type HeLa cells, but not in the XBP-1 knockout HeLa cells. KO Validation Recombinant Protein Test (Figure 3) : Anti-XBP-1 antibodies (3687) detected human XBP-1 recombinant protein at different concentrations. Recombinant Protein Test3687

Signal Transduction, Chemokines & Cytokines

XBP-1 Antibody is affinity chromatography purified via peptide column.

Cat. No. 1414 – Mouse Pancreas Tissue Lysate

1 mg/mL

Predicted species reactivity based on immunogen sequence: Bovine: (100%)

XBP-1 Antibody is supplied in PBS containing 0.02% sodium azide.

None

Blue Ice

XBP-1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IgG

Human XBP-1 has two isoforms, including isoform 1 (261aa, 29kD) and isoform 2 (376aa, 40kD) . Mouse XBP-1 also has two isoforms, including isoform 1 (267aa, 29.6kD) and isoform 2 (371aa, 40kD) . And Rat XBP-1 has two isoforms as well, including isoform 1 (267aa, 30kD) and isoform 2 (371aa, 40kD) . 3687 can detect human, mouse and rat.

XBP1

Cat. No. 1464 – Rat Liver Tissue Lysate

X-box binding protein 1

Homo sapiens

Yoshida et al. Cell 2001; 107:881-91.

Calfon et al. Nature 2002; 415:92-6.

Haze et al. Mol. Cell. Biol. 1999; 10:3787-99.

Little et al. Crit. Rev. Eukaryot. Gene Expr. 1994; 4:1-18.

PMID:19363290

PMID: 19808675

XBP-1 Antibody: XBP2, TREB5, XBP-1, XBP2, X-box-binding protein 1, Tax-responsive element-binding protein 5

ELISA, WB, ICC, IHC-P, IF

60416406

Liquid