YKT6 cDNA

YKT6 is one of the SNARE recognition molecules implicated in vesicular transport between secretory compartments. It is a membrane associated, isoprenylated protein that functions at the endoplasmic reticulum-Golgi transport step. This protein is highly conserved from yeast to human and can functionally complement the loss of the yeast homolog in the yeast secretory pathway.

7p15.1

Human

PATGen (puc19-derived cloning vector)

ATGAAGCTGT ACAGCCTCAG CGTCCTCTAC AAAGGCGAGG CCAAGGTGGT GCTGCTCAAA GCCGCATACG ATGTGTCTTC CTTCAGCTTT TTCCAGAGAT CCAGCGTTCA GGAATTCATG ACCTTCACGA GTCAACTGAT TGTGGAGCGC TCATCGAAAG GCACTAGAGC TTCTGTCAAA GAACAAGACT ATCTGTGCCA CGTCTACGTC CGGAATGATA GTCTTGCAGG TGTGGTCATT GCTGACAATG AATACCCATC CCGGGTGGCC TTTACCTTGC TGGAGAAGGT ACTAGATGAA TTCTCCAAGC AAGTCGACAG GATAGACTGG CCAGTAGGAT CCCCTGCTAC AATCCATTAC CCAGCCCTGG ATGGTCACCT CAGTAGATAC CAGAACCCAC GAGAAGCTGA TCCCATGACT AAAGTGCAGG CCGAACTAGA TGAGACCAAA ATCATTCTGC ACAACACCAT GGAGTCTCTG TTAGAGCGAG GTGAGAAGCT AGATGACTTG GTGTCCAAAT CCGAGGTGCT GGGAACACAG TCTAAAGCCT TCTATAAAAC TGCCCGGAAA CAAAACTCAT GCTGTGCCAT CATGTGA

YKT6, YKT6, ATGD0042-10 µg, ATGD0042-20 µg, ATGD0042-50 µg, ATGD0042-100 µg, ATGD0042-250 µg, ATGD0042-500 µg, ATGD0042-1 mg, ATGD0042-10, ATGD0042-20, ATGD0042-50, ATGD0042-100, ATGD0042-250, ATGD0042-500, ATGD0042-1

Store the plasmid at -20C.

Lyophilized

Signal transduction

606209

NP_006546.1

Human

1. Centrifuge at 7000rpm for 1 minute.; ;2. Carefully open the vial and add 100 µL of sterile water to dissolve the DNA.; ; Each tube contains approximately 10 µg of lyophilized plasmid.

NM_006555.3

597bp

This shuttle vector contains the complete ORF. It is inseted Nde I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.