Zebrafish Actba Antibody / Beta actine

The primary site of action of cytochalasin B on cell motility processes is beta-actin. Habets et al. (1992) generated hybrids that harbor only specific regions of human chromosome 7 and assigned the ACTB locus to 7p15-p12. ACTB and the other assigned beta-actin-related sequences are dispersed over at least four different chromosomes including one locus assigned to the X chromosome. A mutation of beta-actin that alters depolymerization dynamics is associated with autosomal dominant developmental malformations, deafness, and dystonia.

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL

Q7ZVI7

Zebrafish

A synthetic peptide corresponding to a sequence at the N-terminus of zebrafish Beta actin/Actba was used as the immunogen for the Zebrafish Actba antibody.

WB, IHC-P

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This Zebrafish Actba antibody is available for research use only.

After reconstitution, the Zebrafish Actba antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the Zebrafish Actba antibody should be determined by the researcher.

IHC staining of FFPE zebrafish skeletal muscle tissue with Zebrafish Actba antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.