Zebrafish Argonaute 1 Antilichaam / Ago1

Biological Function: Ago1 binds to small RNAs (miRNAs and siRNAs) and guides RISC to target messenger RNAs (mRNAs) for translational repression or degradation. In zebrafish, Ago1 is particularly important for miRNA function during early development, where it helps regulate maternal mRNA clearance and zygotic gene activation. Functional studies have shown that loss of Ago1 impairs embryonic patterning, cell movement, and germ layer formation. Structure and Domains: Zebrafish Ago1 contains the characteristic PAZ and PIWI domains. The PAZ domain anchors the 3' end of small RNAs, while the PIWI domain has RNase H-like activity, enabling mRNA cleavage in certain contexts. Expression Pattern: Ago1 mRNA is maternally deposited and broadly expressed during early embryogenesis. Later, its expression is enriched in proliferative and neural tissues, reflecting its role in controlling gene expression during development and differentiation. Orthology and Conservation: Zebrafish Ago1 is highly conserved with its mammalian counterparts, including human AGO1. This conservation supports the use of zebrafish as a model for studying RNA interference (RNAi), small RNA pathways, and related genetic regulatory mechanisms.

Western blot: 0.5-1 µg/mL

K4I6K9

Zebrafish

An E.coli-derived zebrafish Argonaute 1 recombinant protein (amino acids E377-R410) was used as the immunogen for the Zebrafish Argonaute 1 antibody.

WB

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This Zebrafish Argonaute 1 antibody is available for research use only.

After reconstitution, the Zebrafish Argonaute 1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the Zebrafish Argonaute 1 antibody should be determined by the researcher.

Western blot analysis of Argonaute 1 protein using Zebrafish Argonaute 1 antibody and zebrafish head tissue lysate. The predicted molecular weight of Argonaute 1 is ~97 kDa.