Zebrafish Ascl1a Antibody / Achaete-scute homolog 1

Ascl1a acts downstream of proneural signaling pathways and promotes the commitment of progenitor cells to a neuronal lineage. It is expressed in neural precursor cells during embryonic development and is reactivated in regenerative contexts. In the zebrafish retina and brain, Ascl1a is essential for the regeneration of neurons following injury, making it a prominent factor in regenerative neurobiology. Zebrafish Ascl1a is widely used as a model for studying the molecular mechanisms of neuronal differentiation, stem cell biology, and tissue regeneration. Its expression is tightly regulated by signaling pathways such as Notch, and its function is conserved across vertebrates, including in mammalian neurogenesis.

Western Blot: 0.5-1 µg/mL

Q90259

Zebrafish

E. coli-derived zebrafish Ascl1a recombinant protein (amino acids M1-F196) was used as the immunogen for the Zebrafish Ascl1a antibody.

WB

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This Zebrafish Ascl1a antibody is available for research use only.

After reconstitution, the Zebrafish Ascl1a antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the Zebrafish Ascl1a antibody should be determined by the researcher.

Western blot analysis of Ascl1a protein using Zebrafish Ascl1a antibody and zebrafish embryo tissue lysates. Predicted molecular weight ~22 kDa.