Zebrafish Atp5b Antibody / Atp5f1b / ATP synthase subunit beta

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c) . This gene encodes the beta subunit of the catalytic core.

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL

A8WGC6

Zebrafish

An E.coli-derived zebrafish Atp5b/ATP5F1B recombinant protein (amino acids Q112-S517) was used as the immunogen for the Zebrafish Atp5b antibody.

WB, IHC-P, IF

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This Zebrafish Atp5b antibody is available for research use only.

After reconstitution, the Zebrafish Atp5b antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the Zebrafish Atp5b antibody should be determined by the researcher.

IHC staining of FFPE zebrafish brain tissue with Zebrafish Atp5b antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.