Zebrafish Axin2 Antilichaam / Axis remming eiwit 2

Zebrafish axin2 is dynamically expressed during embryogenesis, particularly in regions undergoing active morphogenesis such as the neural tube, somites, and developing fins. By promoting the degradation of Beta-catenin, Axin2 attenuates Wnt signaling activity and ensures the precise spatial and temporal regulation of target gene expression. Its expression is commonly used as a molecular readout of Wnt/Beta-catenin pathway activity in vivo. Because of its highly conserved role in Wnt signaling, zebrafish Axin2 is widely utilized in studies of developmental biology, stem cell regulation, tissue regeneration, and cancer research. It also serves as an important biomarker for monitoring Wnt pathway modulation in both genetic and pharmacological studies.

Immunohistochemistry (FFPE) : 2-5 µg/mL

P57095

Zebrafish

E. coli-derived zebrafish Axin2 recombinant protein (amino acids H86-D812) was used as the immunogen for the Zebrafish Axin2 antibody.

IHC-P

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This Zebrafish Axin2 antibody is available for research use only.

After reconstitution, the Zebrafish Axin2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the Zebrafish Axin2 antibody should be determined by the researcher.

Cytoplasm

IHC staining of zebrafish Axin2 protein using Zebrafish Axin2 antibody, HRP-labeled secondary and DAB substrate. Axin2 was detected in a paraffin-embedded section of zebrafish spinal cord tissue. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.