Zebrafish Caspase 9 Antilichaam / Casp9

Following mitochondrial outer membrane permeabilization, cytochrome c is released into the cytosol, leading to the formation of the apoptosome complex. Caspase 9 is recruited and activated within this complex, initiating a proteolytic cascade that culminates in the activation of effector caspases such as Caspase 3 and Caspase 7. In zebrafish, casp9 is expressed in multiple tissues during development and is crucial for eliminating damaged, mis-specified, or excess cells. Zebrafish Caspase 9 is widely used in research on cell death mechanisms, neurodevelopment, cancer biology, and toxicology, providing an in vivo model to study mitochondria-mediated apoptosis and the effects of pro- and anti-apoptotic signals. Due to its high degree of functional conservation with mammalian caspase-9, it serves as a valuable tool for both basic and translational research.

Western Blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL

F1QSB1

Zebrafish

E. coli-derived zebrafish Caspase 9 recombinant protein (amino acids K18-Q417) was used as the immunogen for the Zebrafish Caspase 9 antibody.

WB, IHC-P

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This Zebrafish Caspase 9 antibody is available for research use only.

After reconstitution, the Zebrafish Caspase 9 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the Zebrafish Caspase 9 antibody should be determined by the researcher.

IHC staining of FFPE zebrafish eye tissue with Zebrafish Caspase 9 antibody, HRP-labeled secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.