Zebrafish Cdkn1a Antilichaam / p21 / Waf1 / Cip1

In zebrafish, Cdkn1a is expressed in various tissues during embryogenesis and in adult organs, especially in regions undergoing rapid cell division or experiencing genotoxic stress. Zebrafish Cdkn1a is a confirmed ortholog of the human CDKN1A gene, which encodes the protein commonly known as p21. Both zebrafish and human Cdkn1a proteins are structurally conserved and share similar mechanisms of action in regulating the cell cycle and promoting cellular senescence or apoptosis. Cdkn1a is a downstream effector of the tumor suppressor protein p53 and becomes upregulated following DNA damage or oxidative stress. It functions primarily by binding to and inhibiting cyclin E and cyclin A associated kinases, thereby enforcing a cell cycle arrest at the G one and S phase transition. In zebrafish, Cdkn1a is widely used as a biomarker for DNA damage response and is essential for proper embryonic development, tissue regeneration, and maintenance of genomic stability. Due to its evolutionary conservation and central role in cell cycle control and stress response, zebrafish Cdkn1a is a valuable model for studying cancer biology, toxicology, aging, and regenerative medicine. It is often used in research investigating p53 signaling, chemical screening, and genetic regulation of cell cycle arrest.

Western Blot: 0.5-1 µg/mL

A0A8M1RFS1

Zebrafish

E. coli-derived zebrafish Cdkn1a recombinant protein (amino acids M1-Q170) was used as the immunogen for the Zebrafish Cdkn1a antibody.

WB

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This Zebrafish Cdkn1a antibody is available for research use only.

After reconstitution, the Zebrafish Cdkn1a antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the Zebrafish Cdkn1a antibody should be determined by the researcher.

Western blot analysis of Cdkn1a protein using Zebrafish Cdkn1a antibody and 1) zebrafish head tissue lysates and 2) zebrafish embryo tissue lysate. Predicted molecular weight ~15 kDa.